Biochemistry 2280A Lecture Notes - Lecture 28: Genomic Library, Craig Venter, Francis Collins
22 Aug 2016
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Lecture 27 sequencing genomes (ted video richard resnick) Estimated to be billion business within 5 years. Human genome was not the first genome sequenced. Sequencing a genome involves: creating a genomic dna library (cloning, many independent sequencing reactions, aligning the independent sequences into a continuous sequence. Each of these represents a different fragment of dna, but together they represent the whole genome. Libraries of human genomic dna fragments can be constructed using restriction nucleases and ligase. A genomic library comprises a set of bacteria, each carrying a different small fragment of human. For simplicity, only the colored dna fragments are shown ecb3 e10. 23/10. 11 in the library; in reality, all of the gray fragments will also be represented. Dna fragments inserted into plasmids using ligase: influenza genome"s was the first sequenced. The h. influenza genome is 2 million bp. Start with millions of cells of h. influenza and extract the dna.
