Biochemistry 2288A Lecture Notes - Lecture 6: X-Ray Crystallography, Sonication, Absorbance
17 Jun 2016
School
Department
Course
Professor
Document Summary
E. g. human growth hormone, insulin, chemotherapy, auto- immune disorders, beano (a-galactosidase), lactaid (glycoside hydrolase) For use as a reagent in the laboratory. Enzymes for manipulation dna or growth factors to stimulate cells grown in cultures. Proteins are present in cells or secretions, and are thus complex mixtures. Plus other - dna, lipid, small molecules, etc. Must be able to detect in a mixture. Measure some property of the protein of interest. Analytical methods to detect speci c protein of interest. Break open tissue or cells to release protein. Low speed - 100 to 1000 x g (force of gravity( Further separates proteins based on characteristics like charge and size. Proteins in mobile phase interact (or not) with the matrix. Generally a constant ow rate is maintained, and a fraction is collected every few minutes. Usually many fractions from a single run https://uwoca-my. sharepoint. com/personal/akalluma_uwo_ca/_layouts/15/wopiframe. aspx?sourcedoc={5e62ed6a-49ad-486b-a23a-ea4b3ec5cbfc}& l
