Biochemistry 3382A Lecture 4: Dr. Ball's Lecture 4.docx
11 Jan 2015
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It used dntps, mg2+, dna, primer to copy template. There was 3" to 5" exonuclease activity that was part of the enzyme. It also has proofreading to increase the fidelity of the polymerization. However, there were some puzzling things found by other researchers. These include: mutants lacking pol i still survived and replicated dna with poor processivity (10 100 nt). It also had a 5" to 3" exonuclease activity. This means that it degrades dna in the same way that it polymerized it. As a result of these activities you get nick translation by pol i. The pol adds nucleotides to the free 3" end at the nick and at the same time removes all the nucleotides ahead of it. Nick just moves from one position to another position. 3" to 5" polymerase + 5" to 3" exonuclease activities cause a nick to move .
