Biochemistry 3385A Lecture 5: Dna Damage Repair Lecture 5
4 Feb 2015
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1. 3 dna repair: diseases resulting from dna repair deficiencies. Human molecular genetics 2 (mostly chapter 9, some of chapter 18) (available online through uwo. Genes viii, by lewin, b (available online through uwo library: overview of dna mutations. Maintenance of the accuracy of the dna genetic code is critical for both the long- and short-term survival of cells and species. Estimated 108-1010 replication errors per gene per lifetime (estim 10-6 to 10-8 errors/gene/cell division) Among the macromolecules in the cell, dna is the only macromolecule for which mechanisms exist to ensure its integrity. Most common dna-damaging agents, lesions, and repair pathways. Dna crosslink cisplatin (here) environmental mutagens pyrimidine dimers. Uv radiation depurination deamination (uracil) hydrolysis (promoted by acid) Reactive oxygen species (ros) superoxide radical anions, hydrogen peroxide, or hydroxyl radicals formed by- products of oxygen metabolism. Oxidative degradation of the sugar moieties leads to a number of adducts, for example, oligonucleotides with 3"-phosphate ends (14)
