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Lecture 7

Lecture 7.docx

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Western University
Biology 1001A

Lecture 7: Online multiple choice assessment on OWL HHMI animation  Click Question 1: Not mitosis  Distilled Gold   A-T, C-G  Pairing is antiparallel  Extend 3' end of properly paired base  DNA polymerases cannot initiated DNA synthesis, they can only elongate  1 replisome replicates both strands at the same time   1 replisome has 2 DNA polymerase molecules, one replicating each of the two strands  The lagging strand has to be looped to remove the Okazaki fragments  Chromatids are identical DNA molecules attached at their centromeres  List of mechanisms to ensure inheritance of sameness   Complementary base pairing during replication  Old cells die  Checkpoints G1, G2 and metaphase checkpoint  What about the last bubble on the end of a chromosome?   Telomerase brings its own template and adds on the 3' end because that's what polymerase can do   telomerase is based on RNA (RNA template) to synthesis DNA  It just adds more DNA to the 3' end called the telomere (non coding essential DNA), same DNA over and over again  3' telomere end actually bends and creates a cap that protects the end of a chromosome  chromosomes do shorten after each replication  Clicker Question 2: Yes, on the opposite strand  Group On   When/where are cells actively cycling in birds?   Gametes  stem cells  digestive system  Bone marrow (blood)  Liver  Any tissue that needs to be replaced/repair  During early development  When/where are actively cycling cells in a tree?   Meristems/any growing tip  Roots  Shoots/any growing tip  growing leaves  a new born cell might be produced to do its function, to just stay there or programmed to die  When/where might cells be programmed to die (apoptosis)?   Too old, too many mutations   die before it turns into a tumor  cells know how old they are by noticing how long their telomeres are  when every you produce surplus cells, they might die because we don't need it  Sple
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