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Zab Methods Paper.docx

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Western University
Biology 1001A
Tom Haffie

MethodsThree experiments were conducted to determine the effects of different ion sizes222CaCl Ca BaClBa and MgClMgon the transformation of Escherichia 22 2 coli K12 by p600 12gLOne molar of BaCl was diluted using 100 L of 1 M BaCl and 900 L of water 22to make 1000 L of 100 mM BaCl Using a P1000 micropipette 500 L of 100 mM 2CaCl BaCl and MgCl were added into 3 different 15 mL eppendorf tubes A line of E 222coli K12 from a plate was scraped using a sterile toothpick into each of the 15 mL eppendorf tubes All tubes were placed in ice for 2 minutes then each tube was split into two tubes with 250 L in each tube Ten microlitres of TE TrisEDTA was added to 3 tubes of each ionic solution Ten microlitres of p600 12 gL was added to the other three tubes The six tubes were placed in ice for 5 minutes heat shocked at 45
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