Biology 1001A Lecture Notes - Pipette, Electrolyte, Toothpick
21 May 2014
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Three experiments were conducted to determine the effects of different ion sizes. Cacl2 (ca2+), bacl2 (ba2+), and mgcl2 (mg2+) on the transformation of escherichia coli k12 by p600 (12 g/ l). One molar of bacl2 was diluted using 100 l of 1 m bacl2 and 900 l of water to make 1000 l of 100 mm bacl2. Using a p-1000 micropipette, 500 l of 100 mm. Cacl2, bacl2, and mgcl2 were added into 3 different 1. 5 ml eppendorf tubes. A line of e. coli k12 from a plate was scraped using a sterile toothpick into each of the 1. 5 ml eppendorf tubes. All tubes were placed in ice for 2 minutes then each tube was split into two tubes with 250 l in each tube. Ten microlitres of t. e. (tris-edta) was added to 3 tubes of each ionic solution. Ten microlitres of p600 (12 g/ l) was added to the other three tubes.
