Class Notes (835,611)
Canada (509,286)
Biology (6,794)
Biology 1002B (1,346)
Lecture 9

lecture 9.docx

2 Pages
118 Views
Unlock Document

Department
Biology
Course
Biology 1002B
Professor
Denis Maxwell
Semester
Winter

Description
Lecture 9: Saturation Curve 1. How to measure carbon fixation in Chlamydomonas  How much CO i2 actually being incorporate by photosynthesis – amount of CO2produced by respiration  CO 2nalyzer + lights o CO fixation rate (micromol carbon/cell or chlorophyll/min) 2 o Look at concentration change when light turns on or off 2. How one can distinguish between gas exchange in mitochondria from that taking place in the chloroplast of a Chlamydomonas cell.  Measure it in the dark  Measure the level of CO2concentration due to mitochondria 3. Identify major parts of a light response curve for carbon fixation  In the dark o Photosynthesis is off (behave like animal cell) o Rate of mitochondrial respiration only (linear) o Negative CO2fixation since C2 is produced  Room light o Linear increase of CO 2  limit by NADPH, ATP, light  Rate of calvin cycle is directly proportional to rate of the light reaction o More light = more photosynthesis = more CO 2 o More reductant and ATP needed to fix CO2  Low light rate slower (that’s why linear)  Outside o Enzymes in the calvin cycle can’t work any faster (max rate) o Lots of reductant and ATP however rate is limited o Rate plateaus (level off) because of turnover rate of enzymes and regeneration of RuBP  Full sunlight o Max rate reached  Light is excess once saturation point is reached o Protein attached to P680 breaks down/oxidized  Since P680+ is reactive and causes to pull an electron away from protein since it cannot use excess light  Need a lot of protein synthesis to keep the photosystems to work 4. What metabolic processes and external factors may influence the change in rate as a function of light.  Adding or removing reactants o The amount of light
More Less

Related notes for Biology 1002B

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit