Biology 1202B Lecture Notes - Frameshift Mutation, Silent Mutation, Nonsense Mutation
30 Apr 2012
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The basis of many of these methods are denaturing the dna, then trying to find a complementary sequence. Total rna extracted from a cell: 80% rrna, 10-20% trna, 1-5% mrna. Rna, bottom is cdna: rnase enzyme removes the unstable rna to generate a single stranded cdna, this cdna can now go through all the techniques we can do, and it is stable. Cy5 = red dye (experimental group: mix the two single stranded together and allow them to try and find complementary sequences on the membrane (microarray) Rna is very tissue specific, so the rna being examined will be different from organ to organ, or depending on what the person ate or drank before almost anything can change the rna\ Could have a lethal effect or dangerous consequences: silent mutation the mrna changes, but there is no change in the amino acid, frameshift mutation insertion/deletion of a base pair.
