Biology 1202B Lecture Notes - Anemia, Prokaryote, Nijmegen Breakage Syndrome
2 Jun 2012
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1958 meselson & stahl: bacteria grown for several generations in 15n (heavy) medium. Bacteria transferred to 14n (normal/light) medium & al- lowed to grow & divide for several generations. Took the bacteria, known isotope of nitrogen,took it and grew in heavy nitrogen (15) Nitrogen because its a part of the nucleic acid, when they grew bacteria ( sever- Al generations), they found that ll the dna was heavy: i. e they are at the bottom, in n 14 all dna was light. Predicted results: conservative: one --> 14n, one 15 n two ---> 3 of 14 n. 1 of 15 n dispersive: each a mixture of 15 n and 14 n two: misture of 14 n and 15 n but more of. Therefore, it was proven that semiconser- vative method was used for dna repli- cation. * look at dna thesis pic ty of the template is v. important, the top = 3" and is 5", replication occurs 5" to 3".
