Biology 2382B Lecture Notes - Lecture 7: G1 Phase, G2 Phase, Sister Chromatids

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Cell cycle and phases: cell cycle is the ordered sequence of events in which a cell duplicates its chromosomes and divides into two genetically identical cells. Four phases: g1, s (dna synthesis), g2 and m phase. G1 phase: higher % of cells are in g1, ~11h in mammalian cells. Enter into g0 pause if conditions are bad. Preparation for chromosome segregation and cell division (~4h) Chromatin condensation: nuclear envelope breakdown, decondense and reformation of intact nuclei. Dna content is proportional to the amount of hoechst blue stain: twice the amount of dna in g(cid:883) (cid:523)cell dna doubled but haven"t divided(cid:524) Most cell lines go through cell cycle with in 24 hours. Cell cycle times vary for different cell types. Metaphase: chromosomes align in plane center of cell. Anaphase: sister chromatids separate, pulled towards spindle poles. Cytokinesis: separation of membrane: high accuracy and fidelity is required to assure that the chromosomes will be segregated properly.

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