Biology 2382B Lecture Notes - Lecture 2: Plasmid, Point Spread Function, Microtubule
13 Feb 2017
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Document Summary
Anton van leeuwenhoek created first microscope (magnification of 200x) Condenser lens to focus light on specimen. Objective lens to collect light after it has passed through specimen. Ocular or eyepiece lens to focus image onto eye. Typical light microscope magnification is 40x -1000x. Only structures with a high refractive index (ability to bend light) are observable. Resolution - the ability to distinguish between two very closely positioned objects as separate entities. Conventional light microscope usually cannot resolve objects/ cellular features that are less than 0. 2 mm (or 200 nm) apart. N - refractive index of medium between the specimen and the objective lens. Angle of the light entering objective (the bigger the angle, the better because more light) When achieving optimal resolution as defined by d, you want d to be a low number. High resolution does not mean high d value, it means better quality. A better objective will let in a lot more light.
