Biology 2382B Lecture Notes - Lecture 9: Paclitaxel, Colchicine, Stathmin

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The cytoskeleton is an intricate network of protein filaments that extend throughout the cytoplasm. Movement inside to the cell aided by the cytoskeleton. Polymer of a and b tubulin, tubulin makes microtubules, the dimer is very stable and never unmade. The dimer can be denatured but dimer in functional state. Monomers 55kda each a b dimer is basic subunit . Have polarity, one side different from the other, a (- end) vs b (+ end) Protofilaments come together to form a tube which is hollow. The seam is where things don"t line up properly. Dimer is very stable a binds permanently to gtp, b can hydrolyze gtp so can be bound to gtp or gdp. As the polymer (protofilament) grows, b"s gtp is hydrolyzed. Singlet (cytoplasm) most common, 13 protofilaments form a tube of. Doublet (cilia and flagella) 13 protofilaments with a tube of 10 attached. Triplet (basal bodies and centrioles) 13 protofilaments with two tubes of 10 attached.

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