Biology 2382B Lecture 2: Cummings Lecture 2-Imaging in Cell Biology
16 Aug 2017
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Chapter 4: p. 135-136 (monoclonal antibodies), 139-149, 156-161 (em) First to observe living protazoa and bacteria which he called animalcules . Went on to visualize human red blood cells and sperm. With great skill at grinding lenses, naturally acute eyesight, and lots of patience, he was able to achieve a magnification of 200x. Condenser lens focus light on specimen. Objective lens magnifies light after it has passed through specimen: 3 different objective lenses in picture with 3 different magnifications. Ocular or eyepiece lens focus image on eye: magnifies 10-fold. Compound microscope b/c more than one lens. Total magnification is the product of the magnification of the objective lens and the ocular lens. In order to see detail in your specimen, you need to have contrast. Achieved by refractive ability of specimen: refraction ability to bend light/slow light down; important for seeing detail in a specimen. Some parts of the cell are more refractive than others.
