Biology 2382B Lecture Notes - Lecture 3: Hoechst Stain, Cell-Cycle Analysis, Fluorophore
2 Feb 2018
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Lecture #3: isolation and analysis of cell organelles and molecules. The greater the fluorescence, the greater the charge: fluorescent cell droplets with different electric charge are separated by an electric field and collected. Cell droplets that lack fluorescence and charge are also separated. Therefore, cells that lack the specific antibody/protein are separated. Dna (double the stain) but the cells have not yet divided. How are proteins separated from the organelles: proteins are separated from an organelle with the use of detergent, amphipathic molecules, non-ionic detergents disrupt the lipid bilayer, ex. Integrate into membranes and create pores through which proteins found within the membrane are released but their native conformation is still in tact. Sds-page: electrophoretic separation of proteins is most commonly performed in polyacrylamide gels and called polyacrylamide gel electrophoresis (page, page is usually carried out in the presence of the negatively charged detergent.
