Biology 2382B Lecture Notes - Lecture 16: Embryonic Stem Cell, Petri Dish, Trypsin
5 May 2018
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Embryonic Stem Cells
1) Zygote
2) Cleavage Stage Embryo
3) Culture Blastocyst (contains inner cell mass)
- isolate cells from inner cell mass + place them on a
petri dish containing fibroblast FEEDER cells (excrete
factors and proteins that allows survival + proliferation
of embryonic stem cells in undifferentiated state)
4) Surviving cells are then isolated using trypsin to dissociate them
5) Plate individual cells with more feeder cells and will adhere and
start to form columns
- Now called Embryonic Stem Cell Lines (undifferentiated)
- Type of cell line that is NOT transformed!
6) Now can grow indefinitely (very hard!) OR embryoid bodies can
differentiate into any cell type
To differentiate put in suspension (w/specific growth factors), will
form Embryoid Bodies, + can manipulate into the 3 Germ Cell Layers:
A) Endoderm (ex. Gut Epithelium)
B) Mesoderm (ex. Cartilage)
C) Ectoderm (ex. Neural Precursors)
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Document Summary
Isolate cells from inner cell mass + place them on a petri dish containing fibroblast feeder cells (excrete factors and proteins that allows survival + proliferation of embryonic stem cells in undifferentiated state) Embryonic stem cells: zygote, cleavage stage embryo, culture blastocyst (contains inner cell mass, surviving cells are then isolated using trypsin to dissociate them, plate individual cells with more feeder cells and will adhere and start to form columns. Now called embryonic stem cell lines (undifferentiated) Type of cell line that is not transformed: now can grow indefinitely (very hard!) Or embryoid bodies can differentiate into any cell type. To differentiate put in suspension (w/specific growth factors), will form embryoid bodies, + can manipulate into the 3 germ cell layers: endoderm (ex.
