Biology 2382B Lecture Notes - Lecture 1: Cell Culture, Ethylenediaminetetraacetic Acid, Trypsin
24 Oct 2018
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Recommended readings from the textbook: chapter 1: p. 12 , chapter 4: p. 132; chapter 21: p. 975- 977, 980-985; 991-992 ; chapter 24: p. 1140. Why study cells: part of the core in biology, fundamental unit of life, many unanswered questions. Storehouse for thousands of genes: determine what is (cid:498)normal(cid:499) so we can fix the (cid:498)abnormal(cid:499, last century was the century of the gene. In order to figure out what the genes do, you have to put it in context to the cell. What is a cell: mitochondria acts as a signalling molecule. In vitro: in artificial environment outside of the body in culture. Cell culture is the technique used to grow cells or tissues outside the organism under strictly controlled conditions. Cells are isolated from any tissue by breaking down the cell-cell and cell-matrix interactions (use mechanical fragmentation, trypsin, edta)
