Biology 2382B Lecture Notes - Hela, Primary Cell, Embryonic Stem Cell

• School: Western University
• Department: Biology
• Course: Biology 2382B
• Professor: Robert Cumming

Document Summary

2+ as calcium is integral in binding to membrane proteins that make cells more cohesive): once dissociated, cells need to be maintained at favourable conditions in a specialized. , which is specifically added to indicate the culture"s ph: acidic cultures arise when too many cells are present resulting in a production of lactic acid that turns the media yellow. Roller bottles can also allow for a suspension cell culture to grow by mechanical agitation. In addition to senescence, cells also undergo contact inhibition (where cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing) if cell density is high. Senescent cells are related to ageing: a cell line refers to cells which are transformed and are able to grow indefinitely. Birth, lineage & death of cells: the origin of all bodily cells can be traced back to stem cells (whether embryonic or adult). *stem cells contribute no real function (except making differentiated cells), while differentiated cells do.