Biology 2382B Lecture Notes - Lecture 11: Intermediate Filament, Cell Adhesion, Tubulin

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Microfilaments: subunit actin, diameter 7-9nm. Microtubules: subunit alpha-beta tubulin dimer, diameter 25nm. Intermediate filaments: various subunits, diameter 10nm, *know a lot less about intermediate filaments. The microtubules have polarity one side different than the other: designated by + end and end, not an electrical charge, one side is called + because that is where most of the growing occurs. Always add the apha beta dimers in the same order alpha beta, alpha beta, etc. Side is always alpha, and the + side is always beta. Protofilament is the end to end attachment of the dimers: also has polarity one side is and the other is + Protofilaments come together to form a hollow tube, which is the microtubule. The alpha tubulin monomer binds permanently to gtp: cannot hydrolyze it. The beta tubulin monomer can hydrolyze gtp, so it can be bound to both.

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