Biology 2382B Lecture Notes - Lecture 1: Scanning Electron Microscope, Fluorophore, Tubulin
18 Jun 2020
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Why study cells: most living things are composed of cells, disease mechanisms are usually at cellular level, i. e. abnormal cell state = disease. What is a cell: eukaryotes have plasmic & nuclear membranes. Symmetric and asymmetric cell division: symmetric cell division two identical daughter cells, good for experimental purposes, asymmetric cell division two differentiated stem cells or one differentiated stem cell. Pluripotent stem cells can be obtained from differentiated. Resolution (d) of microscopes: resolution smallest distance between two objects where they can be distinguished, smaller resolution = more detail, nsina = numerical aperature, n = refractive index of medium between objective lens. & specimen: a = angle of light entering objective, min. distance we can distinguish objects is 0. 2 m, oil aversion oil has a higher refractive index which increases denominator. Wavelength spectrum used in microscopy: visible light = 400-700 nm light microscopy = 300-700 nm, fluorescence microscopy = 300 nm, electron microscopy = 1 nm.
