Biology 2382B Lecture Notes - Lecture 2: Vacuole, Chromophore, Hat Medium
4 Jul 2020
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Document Summary
Features of a modern compound microscope: bright-field microscopy (aka compound microscope, live cells, light source, 3 lenses, condenser lens -> to focus light on specimen. If there"s 2 parallel wavelength and one is shifted by. (wavelength), it will cause interference: shift light can produce interference -> producing dimmer lights, which is why specimens look darker. Shift: exploiting that shift to make another shift. Interference between polarized light generates contrast: defines outlines of large organelles and provides better detail of cell edge, ex: nucleus and vacuole, polarized light is scattered to give a 3d image. Fluorescence microscopy: uses property of certain molecules to emit visible light when they absorb light at a specific wavelength, has bright field microscopy to find the sample then uses fluorescence, has special lights and filters. Location of fluorescent protein molecules or dyes can be imaged: fluorochromes -> fluorescent dyes, absorb energy, excites e- into higher orbital (unstable)
