Biology 2382B Lecture Notes - Lecture 10: M60 Machine Gun, Metaphase, Microtubule
29 Feb 2016
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Mpf when active profoundly changes the cell (g2-m) don"t wanna activate till ready. Cyclin starts to increase before m, cyclin starts binding cdk to make mpf don"t wanna activate mpf till ready. As youre getting more and more cyclin wanna start activating things, Remove phosphate to activate using cdc25 (removing inhibition) but not enough, need more activation. When cell is ready activates cak which activates t161. Then active mpf has no phosphorylation on t15, and phosphorylation on t161. Mpf now actice it"s a kinase phosphorylates many things: remember underlined things (changes in cell, mpf is active b/c cfk is active. Apc name of ubiq ligase that is activated just after metaphase and triggers degredation of cyclins. E3 is the ubiq ligase present in most cells recognizes specific domains (destruction box present in all cyclins) Active mpf functions till metaphase apc regonizes cdks degrades. Mpf made of mitotic cyclin and cdk.
