Biology 2581B Lecture Notes - Lecture 21: Telomere, Dosage Compensation, Retrotransposon
21 Mar 2016
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She looked at colour formaion of maize to io stuf b/c of jumping you gain or lose colour. Slide 7: transposable elements are diferent based on species but exist in mammals, insects, plants diferent copy numbers depending on element and species not reliant on anything else. In drosophila require certain transposable elements to maintain telomere length. 45% we can associate with jumping genes in our genomes percentage based on sequences/nts per genome. Slide 11: non-ltr oten have poly a tail like thing (but looking at dna level not rna) Ltrs have direct long terminal repeats surrounding. Experiment to prove they need rna intermediate to move from one locaion to another. Use a plasmid shutle plasmid can gro win e coli and yeast. In plasmid cloned whole yeast ty1 retrotransposon (green) Part of that is coding sequence for revere transcriptase and part of it is ltr. Altered it further inserted intron into coding region disrupts coding sequence.
