Biology 1002B Lecture Notes - Lecture 14: Repressor, Cyclic Adenosine Monophosphate, Spliceosome

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blushporpoise372

30 Mar 2017

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All rna is made by transcription, during transcription the rna complimentary base pairs with. Not possible for amino acids to base pair with anything. If the repressor is bound to the operator, the promoter cannot get through (negative control) Cap/camp binding protein complex binds upstream of the promoter and increases expression (positive control) Glucose transport into the cell reduces camp no longer available to bind with cap, no longer able to bind to dna, expression will fall. Positive/ negative control depends on effect of dna binding protein. Mutant who"s galactosidease activity does not rise fast enough when lactose is added and does not fall off when glucose is added: Lac l cap binding site promoter operator lac z. Gene structure in the nucleus is more complex than in organelles. At rich segment of gene called tata box, attracts transcription binding protein and additional general transcription factors to attract rna polymerase to begin transcription.

Related Questions

When (glucose, lactose) ___________ levels inside a bacterial cell are low, cyclicAMP levels are (high, low) __________. This is because metabolizes sugars (activate, inhibit) ___________ the enzyme adenylate cyclase, and (activate, inhibit) __________ the enzyme cAMP phosphodiesterase, causing cyclic AMP levels to rise. Consequently, the CAP protein is (Activated, inhibited, unaffected) ___________ by the cyclic AMP and binds to the lac promoter region, and the CAP protein acts like a sigma factor recruiting (DNA, RNA) ___________ polymerase to the promoter. This establishes condition that may lead to the (transcription, replication) __________ of the lac operon if (allolactose, glucose, lactose) _____________ is available to bind to (lac repressor, lacZ, lacY, lacA) _________ protein keeping this protein from binding to the (lacO, lacZ. lacY, lacA) ___________ gene.

yellowwasp623

1. What is the term when the product of a biochemical pathway inhibits the activity of the first enzyme of a pathway?

		feedback inhibition.
		activator inhibition.
		diffusional regulation.
		enzyme activation.

2. Through control of gene expression, prokaryotes respond to changing __________ conditions.

		internal
		protein
		environmental
		genetic

3. A protein that negatively regulates transcription by binding to the operator is known as the

		operon
		repressor
		activator
		inducer

4. During attenutation, when tryptophan levels are high, the ________ stem-loop forms and transcription ________ the trpL gene.

		1-2, ends just past
		3-4, ends just past
		1-2, continues beyond
		3-4, continues beyond

5. The process of turning on the expression of a gene in response to a substance in the environment is called:

		constituation.
		repression.
		induction.
		catabolism.

6. What is a function of some specific transcription factors?

		to bind to the promoter region of the gene to facilitate the proper alignment of the RNA polymerase
		to bind to the centromere to induce meiosis
		to bind to enhancer regions to help regulate gene transcription
		to bind to the operator to recruit sigma factor

7. How does exposing an E.coli cell to glucose affect the regulation of the lac operon via CAP?

		cAMP binds to CAP and transcription is increased.
		cAMP binds to CAP and transcription is decreased.
		cAMP does not bind to CAP and transcription is increased.
		cAMP does not bind to CAP and transcription is decreased.

8. The lac repressor protein works when mutations in the repressor gene and structural genes are:

		in cis or in trans.
		in trans only.
		in cis only.
		in both prokaryotes and eukaryotes.

scarletwombat949

Determine the outcome for the lac operon genotype shownbelow:

I^sP⁺O^cZ⁺

Assume lactose is present.

Select one:

Functional beta-galactosidase is produced constitutively.

Functional beta-galactosidase is NOT produced.

Determine the outcome for the lac operon genotype shownbelow:

I^-P⁺O^cZ⁺

Assume lactose is present.

Select one:

Functional beta-galactosidase is produced constitutively.

Functional beta-galactosidase is NOT produced.

Mark all that are true about the lac operon.

Select one or more:

CAP and cAMP bind to increase transcription of structuralgenes.

RNA polymerase binding occurs best if CAP is present and cAMP isabsent.

The presence of glucose decreases CAP-cAMP binding to theoperon.

The presence of additional cAMP decreases CAP binding to theoperon.

DNA methylation may be a significant mode of genetic regulationin eukaryotes. Methylation in this case refers to...

Select one:

Addition of methyl groups to RNA polymerase

Addition of methyl groups to tRNA

Addition of methyl groups to DNA polymerase

Addition of methyl groups to cytosine

amethystllama616

Biology 1002B Lecture Notes - Lecture 14: Repressor, Cyclic Adenosine Monophosphate, Spliceosome

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