When (glucose, lactose) ___________ levels inside a bacterial cell are low, cyclicAMP levels are (high, low) __________. This is because metabolizes sugars (activate, inhibit) ___________ the enzyme adenylate cyclase, and (activate, inhibit) __________ the enzyme cAMP phosphodiesterase, causing cyclic AMP levels to rise. Consequently, the CAP protein is (Activated, inhibited, unaffected) ___________ by the cyclic AMP and binds to the lac promoter region, and the CAP protein acts like a sigma factor recruiting (DNA, RNA) ___________ polymerase to the promoter. This establishes condition that may lead to the (transcription, replication) __________ of the lac operon if (allolactose, glucose, lactose) _____________ is available to bind to (lac repressor, lacZ, lacY, lacA) _________ protein keeping this protein from binding to the (lacO, lacZ. lacY, lacA) ___________ gene.
When (glucose, lactose) ___________ levels inside a bacterial cell are low, cyclicAMP levels are (high, low) __________. This is because metabolizes sugars (activate, inhibit) ___________ the enzyme adenylate cyclase, and (activate, inhibit) __________ the enzyme cAMP phosphodiesterase, causing cyclic AMP levels to rise. Consequently, the CAP protein is (Activated, inhibited, unaffected) ___________ by the cyclic AMP and binds to the lac promoter region, and the CAP protein acts like a sigma factor recruiting (DNA, RNA) ___________ polymerase to the promoter. This establishes condition that may lead to the (transcription, replication) __________ of the lac operon if (allolactose, glucose, lactose) _____________ is available to bind to (lac repressor, lacZ, lacY, lacA) _________ protein keeping this protein from binding to the (lacO, lacZ. lacY, lacA) ___________ gene.
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Related questions
QUESTION 1
In the bacterial lac repressor, which of these occurs: (select all that apply)
| (a)In the presence of glucose and the absence of lactose, the repressor is actively produced. | ||
| (b)The lac operon can be hyper-induced in the presence of low glucose by the 'cAMP binding protein' (CAMP). | ||
| (c)In the presence of lactose and the absence of glucose the lac operon is repressed. | ||
| (d)Allolactose is a byproduct of β-galactosidase, indicating high lactose when it builds up. |
1.5 points
QUESTION 2
In eukaryotes the enhancer elements can be quite a distance upstream or downstream from the promoter, and transcriptional regulation is helped by protein:protein interactions.
True
False
1.5 points
QUESTION 3
The prokaryotic promoter contains a TATA box that is at -10bp upstream from the transcriptional start site.
True
False
1.5 points
QUESTION 4
During the addition of the poly A tail which of these occurs:(select all that apply)
| (a)The transcript is synthesized beyond the poly A site (AAUAAA). | ||
| (b)The mRNA contains a 5â cap. | ||
| (c)The transcript is cleaved 35nt downstream from the poly A site. | ||
| (d)An enzyme complex containing an exonuclease and polyadenylate polymerase cleaves nearby the AAUAA site. | ||
| (e)100-300 Aâs are added to the 5â end. |
