Biology 1002B Lecture Notes - Lecture 21: Luciferase, Mutation, Volvox
11 Apr 2017
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Strategies for annotation of genome sequence (finding orfs) Used to visually see where a gene is being turned on in an organism. Gfp (green fluorescent protein) fluoresces green under uv light lacz expressed in blue. Luciferase enzyme fireflies use to make light. Itr where gene gets integrated into the host gene. Efi alpha gene elongation factor produced strongly in all cells (strong promoter) Chr2 channelrhodopsin; fused with eyfp (yellow fluorescent protein) Wpre from the woodchuck virus gene, 3" utr element, makes message resistant to degradation hgh (poly a) human growth hormone. Exome all sequences that are expressed in a genome. Strategies for confirming that a given orf is actually a gene. Computer looks for start codon in frame stop codon. 6 possible reading frames (3 on top strand, 3 on bottom strand: 6 different places where a gene might be coded. Not a lot of space in prokaryotic genomes for these genes: genes are very dense.
