Biology 1002B Lecture Notes - Lecture 20: Reverse Transcriptase, Antimicrobial Resistance, Unicellular Organism

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Basic mechanism of propagation of action potentials in neurons. Movement of positive ions across the nerve membranes causes depolarization; results in action potential which is successive waves of polarization. Sodium ions can get through the membrane; membrane is permealized such that sodium can enter and depolarize the membrane potential. How channelrhodopsin can control action potentials in neurons. In chlamy, channelrhodopsin allows + ions to enter cell with blue light. Shine light, results in action potential, + ions flow in and membrane is depolarized and fired. Possible sources of genes (ie. channelrhodopsin) for use in making transgenic organisms. Chop out the gene for channelrhodopsin from chlamy using restriction enzymes. Use reverse transcriptase from the mrna of chlamy channelrhodopsin to make cdna: think about difference between genomically derived genes and cdna, there would be no introns in cdna; no promoter either. Give the sequence to a dna printer: gene machines need a relatively short sequence; they"re limited.

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