Biology 1002B Lecture 21: BIO1002B, lec 21
Document Summary
Pcr ampli es (makes billions of copies of a speci c dna sequence) copy number of a speci c sequence. Taq polymerase needs to be not denatured at high temperatures because pcr relies on thermal cycles and we need an enzyme that is stable through the temperature cycling. Notice: these are dna primers we are priming dna with dna (we have only discussed rna primers before) By cycle 3 we have double stranded dna and the molecules we want increase exponentially until they are all that is there. Various technologies deliver transgenes to nuclei or organelles. Examples of transgenics : basic research, commercial products/industrial production, medical treatments. Bacterial photography: a transgenic circuit detects light and deposits black pigment. Made e. coli able to detect light by inserting two genes which allow it to make pcb. Light sensitive protein was external, needed an internal signal as well .
