Biology 1002B Lecture Notes - Lecture 11: Cellular Respiration, Light-Independent Reactions, Oxygen Evolution

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Measuring metabolism is complicated because the oxygen is made by the chloroplasts, but is used by the mitochondria, and vice versa for carbon dioxide. Measuring photosynthesis: gas exchange rate of oxygen evolution and carbon dioxide consumption for photosynthesis, and vice versa for cellular respiration. Measuring the amount of co2 in the chamber to measure the rate at which it is being fixed. Particularly interested in the role of light what is the relationship between the rate of. Measuring photosynthesis photosynthesis and the amount of light available. Rate of co2 fixation = (micromole co2 consumed)/min/cell: more cells = greater rate of fixation therefore, must standardize the number of cells, could also measure per microgram chlorophyll (or something similar; just need to standardize it) Respiration is always occurring (the rate is -8) there is a point where the rate at which the mitochondria are releasing co2 is balanced with the rate at which the co2 is being fixed light compensation point.

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