Biology 1002B Lecture Notes - Lecture 9: Enzyme Kinetics, Allosteric Regulation, Competitive Inhibition
3 Feb 2015
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Hard to measure how much co2 is being brought in by photosynthesis because cellular respiration also producing co2. To measure co2, chlamy cells put into co2 analayzer and measures change in co2 levels. Co2 fixation rate = conversion of co2 incorporated into rubp. How one can distinguish between gas exchange in mitochondria from that taking place in the chloroplast of a chlamydomonas cell. Y-axis represents net fixation of co2, incorporates co2 released from mitochondrial respiration. Dark (no light), fixation rate for co2 for chlamys will be negative, photosynthesis is off and is acting like an animal cell producing co2 not making it therefore negative. Have a chamber with chlamy and change the light. Identify major parts of a light response curve for carbon fixation. Room light, amount of co2 fixation increases linearly; more nadph, atp in order for calvin cycle to fixate. The more nadph, atp, the more the rate will increase.
