Biology 1002B Lecture Notes - Polyadenylation, Transfer Rna, Release Factor

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5 Apr 2012
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Signals
Prokaryote/
Eukaryote?
Signal
Function
Recognized
As:
Recognized
By:
Both
Promoter
Attracts transcription factors that
attract RNA polymerase
DNA
Proteins/
RNA
polymerase
Eukaryotic
Activators
Bind enhancers with promoter
Eukaryotic
TATA Box
Sequence on promoter that recruits
general transcription factors
DNA
Proteins
Eukaryotic
Enhancers
Stabilizes RNA polymerase and
increase transcription rates
DNA
Proteins
Prokaryotic
SD Box
Base pairs to complimentary rRNA to
help mRNA bind to ribosome
mRNA
rRNA
Eukaryotic
Introns/ Exons
Recruit splicesosomes to splice introns
out of pre mRNA; alternative splicing
mRNA
snRNAs
Both
Protein binding
sites (CAP)
Transcriptional Regulation
DNA
Proteisn
Eukaryotic
Cleavage signals
(polyadenlyation)
Recruits RNase to cut the pre mRNA
mRNA
Rnase
Prokaryotic
Terminator signal
Creates hairpin loop that 'knocks off'
RNA polymerase
mRNA
RNA
polymerase
Eukaryotic
Promoter proximal
region
Transcriptional Control
DNA
Proteins
Eukaryotic
5' cap
Translational Initiation
rRNA/
Ribosomes
Eukaryotic
PolyA tail
Protection from denaturation; longer
the tail, more efficient
Eukaryotic
Operator
Regulates trasncription
DNA
Repressor
protein
Both
Start Codons
Translational Initiation
mRNA
tRNA
Both
Stop Codons
Translational Termination
mRNA
Release
factor
Both
Sense Cdons
Translation elongation
mRNA
tRNA or
rRNA
Both
Peptide Signal
Sequence
Tags' proteins to specific locations in
the/ out of the cell
Protein
Peptidase
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