Biology 1002B Lecture Notes - Lecture 14: Guanine, Deoxyribose, Dna Ligase

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[lecture 7 genome replication summary] September 30, 2013: purine and pyrimidine base- pairing in dna/rna. Two of the bases (adenine and guanine) are purines and the other two (thymine and cytosine) are pyrimidines. Purines bond with pyrimidines (a and t, c and g). These relationships are chargaff"s rules. In rna, thymine is replaced with uracil. The base pairing remains the same (a with u) This is complementary base pairing. The deoxyribose sugars are linked by the breaking of phosphate groups. This means dna polymerase can only add molecules at the 3i end (on the hydroxyl group) Because of the anti- parallel nature of dna, the template strand is read in the 3i to 5i end. The other must be replicated in short strands called okazaki fragments away from the replication fork. Leading strand: the new strand of dna synthesized in the direction of unwinding.

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