Biology 1002B Lecture Notes - Lecture 19: Channelrhodopsin, National Center For Biotechnology Information, Optogenetics
26 May 2016
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Take mutants of chlamy that don"t respond to light. Start at gene ends and gure out protein (biochemical approach vs. genetic) No stop at the end of channelrhodopsin************************ Yellow uorescent protein following eyfp, no stop before the chr2. This way, the eyfp is stuck to the chr2, so you know what neurons have the protein. National center for biotechnology information: ncbi is a repository of molecular information (need to isolate genomes here rst always to publish anything) !1: genbank and wgs (whole genome sequences, going to ncbi gets me the actual piece of dna, but not the sequence. !2: primers that are 16 bases long are good enough because the sequence. Monday, march 14, 2016 does not: primers don"t just bind everywhere. You know exactly the gap btwn the primers (pcr product) You know the predicted size of the amplicon. Run pcr reaction on gel, know predicted size, cut band from gel, sequence it, check database sequence.
