Biology 1002B Lecture Notes - Lecture 9: Membrane Protein, Facilitated Diffusion, Enzyme
31 May 2016
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Glucose can be used in photorespiration, which then releases co2. Measuring carbon fixation: co2 analyzer, chamber with chlamydomonas, co2 fixation rate = umol co2 consumed/min/cell. Co2 concentration going up when low light: respiration. When light increased, photosynthesis starts: saturates at around 500. At one point there is an equilibrium of co2 in the cell, the process of the chloroplast (photosynthesis) is matching the process of the mitochondria (respiration: light compensation point (where co2 fixation is 0) Need to be above the light compensation point for plant to grow: carbon fixation plateaus at 500 since the calvin cycle can"t go too fast. Enzymes: e + s (substrate binding) es (catalysis) e + p, temperature sensitive. The amount of substrate needed to reach 1/2vmax is called. Km: affinity: attractiveness between enzyme and substrate. Some e-s graphs don"t need much [s] to get to vmax. Move via membrane protein pores: simple diffusion (dashed)
