Biology 2290F/G Lecture Notes - Lecture 17: Apaf1, Programmed Cell Death, Caspase
20 Apr 2017
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Document Summary
Programmed cell death (apoptosis) is a key process. Active cellular process that is morphologically distinct from necrosis (where cells swell and burst) There are many ways to kill a cell. When apoptosis triggers, cell starts to shrink. Parts of the cell contained in little bags. All these bags recognized by phagocytic cells which engulf them. Apoptotic bodies are encapsulated in vesicles so that the junk doesn"t spread. The dna itself gets cleaved in a repetitive manner. If you were to subject it to gel electrophoresis, you would see a ladder: the things that cut the dna do it at nucleosomes. You get 200bp incremental pieces of dna. Every single cell (959 in adult hermaphrodite and 1031 in the adult male) has been mapped out. We can use dic microscopy to observe this organism. Mutations in the ced-3/ced 4 genes block apoptosis in c. elegans.
