Biology 2290F/G Lecture Notes - Lecture 13: Ph, Acid Dissociation Constant, Photon
5 Jan 2020
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In spectroscopy : read the a400 for the resuspended 1a, 1b and 1c. In these case, #cells/ml = a400 / slope: describe the meaning, and give the units, of photon fluence rate. Measure the amount of photosynthetically-relevant light falling on each reaction mixture at each of the distances from the lamp that we ran the reactions. = moles of photons/m^2/second: know and use the following ph-related equations. [h+][oh-] = 1 10^-14, ph + poh = 14. Ph = - log[h+], [h+] = 10-ph. Poh = - log [oh-] , [oh-] = 10-poh. Consequently, the reactions will be run in the presence of a buffer solution to minimize changes in ph during the course of the reactions. The ph of the environment should lie within 1. 0 ph unit on either side of the pka for that buffer. All components of the buffer should be compatible.
