Biology 3326F/G Lecture Notes - Polyacrylamide Gel Electrophoresis, Coomassie Brilliant Blue, Hsf1
2 Apr 2013
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Document Summary
Cells are exposed to different kinds of stress (environmental, physiological, and pathological) and develop the evolutionary conserved response, which is attributed to the heat shock proteins (hsps). Hsps protect cells from extreme physiological, pathological, and environmental conditions: Serve as molecular chaperones to assist the proper folding of other proteins and to prevent protein aggregation. Directly and indirectly inhibit cell death pathways including apoptosis and interacting with the cytoskeleton maintaining the cell integrity. Hsp 10, 20-30, and 100 interact preferentially with actin microfilaments. Hsp70 with microtubules, microfilaments, and intermediate filaments. Protein family grouped into subfamilies based on their molecular weights. Hsp90: constitutively expressed and act intracellularly as molecular chaperones (preventing premature folding of nascent polypeptides) Hsp27 and hsp70: expressed at low basal levels and increase in response to environmental and physiological stressors. The transcriptional activation of hsp genes is mediated by a specific family of tfs known as heat shock factors (hsf):
