Microbiology and Immunology 2500A/B Lecture Notes - Lecture 24: Reoviridae, Hepadnaviridae, Paramyxoviridae

• School: Western University
• Department: Microbiology and Immunology
• Course: Microbiology and Immunology 2500A/B
• Professor: Dimitrios J Dikeakos, John Mc Cormick, Kelly Lynn Summers

Document Summary

Understanding the various types of genomes found in viruses and how these genomes function. Background on the importance of the genome. Viral classification scheme using the baltimore method. How dna based genomes get their message across. How rna based genomes get their message across. 1950s: established that viral genome is the genetic code. Allow bacteriophage to infect e. coli with radioactive precursors to viral genome or to protein capsule: left radioactively labelled protein capsule, right radioactively labelled viral genome. Blend viruses (capsules) are removed from e. coli in the blender: left bacteriophage infects & inserts its genome in e. coli. Non-radioactive interior: right bacteriophage infects & inserts its genome in e. coli. Separate cells and bacteriophages are separated by centrifugation: take these samples and spin them and use sedimentation course to pellet what is more heavily sedimented on the bottom of the two. Left pellet will not contain radioactivity because it has been pushed away through the blending action (capsule).