Microbiology and Immunology 3300B Lecture Notes - Lecture 33: Elispot, Electrophoresis, Lysis
20 May 2016
School
Department
Professor
Document Summary
Direct coombs test: antibodies that recognize antigens on the surface of blood. 2. if the blood clumps up together, the test is potiive for an antigen. Indirect coombs test: use recipient serum with donor blood recipient serum has antibodies. 2. if you have no clumps, you have the same blood type. Over/underloading: evaporation lines, often have a short window in which result must be read limitations, semi quantitative, sensitivity is moderate, automation is difficult, requires antibody specific to target. Advantages: extremely sensitive high specificity false positive and negative rates are low very quantitative disadvantages radioactivity: modest and manageable risk but requires liscensing, and specially trained people, this is very expensive, requires antibody specific to target. Elisa enzyme linked ig assay enzymes used in place of radioactivity cheaper, safer, but sensitivity is lower the more colour you have, the more the concentration of antigen.
