Microbiology and Immunology 3300B Lecture Notes - Lecture 15: Histology, Fluorophore, Cd19
20 May 2016
School
Department
Professor
Document Summary
The need for single cell analysis herzenberd said his eyes hurt when he was trying to count cells. A teqnique for measuring the physical and chemical properties of cells or microscopic particles suspended in a fluid stream. Data is obtained through laser excitation of fluorescent probes on or within a particle of interest (antibodies, proteins, dyes or probes. Flow cytometric data can either stand alone or can nicely complement other imaging technologies. Large range of particle size (0. 2-100 microns) dust particles to large cells. Quantitative: multiple discrete, simultaneous measurements are made on every single particle: gathered one cell at a time but very high input. High throughput: data is collected for thousands to tens of thousands of events/cells per second. Cells must be in suspension, thus disrupting tissue structure (histology) No information about intracellular localization (confocal microscopy) Histogram: fluorescence on x axis, cell count on y axis.
