Microbiology and Immunology 4100A Lecture Notes - Lecture 1: Translocon, Ion Channel, Membrane Protein
22 Dec 2019
School
Department
Professor
Document Summary
Even if we don"tknowhow it works if wefigureouthowitssecreted we cantarget it using antimicrobials. Formation ofsurfaceappendages flagellaandpilli thatmaythemselvesserve as secretion systemsrequire implementation of basicproteinsortingand secretion systems knowledge ofsecretionsystemsareessentialin a labsetting. Cytoplasm periplasm sec tat innermembranegm srp cytoplasm outermembrane om penplasm. Forms a channel toallowproteinsthroughaslong asthey are unfolded can"tbe inhibited. Is an atpase essential forprovidingtheenergyrequiredtopushthepolypeptide throughthetranslocon via atphydrolysis. Srprecognizes non cleaved n terminalsignalsequence theanchorsequencewhich tethers theproteintotheinner membrane. Srpbindstothe newproteinonce it exitstheribosome theentireribosome is recruitedto thereceptor theprotein is threadedthroughthesecyegchannel as it isbeingsynthesized co translational. Component tat a b c etc all compriseof 1 toseveral in helices system is usedforhigh levelsecretoryexpression of heterologousproteins. Currently acceptedmechanism based on e colicomponents tat a bandc. Tatband tat ccometogether to make a complex that isinvolved in initial rrsignal peptiderecognitionandbinding ofprecursor proteins. Signalpeptidasedeavesthesignalpeptide releasingtheprotein membranedistorted proteingoesthrough influx membrane seared sprsefidase. Folding ishighlyregulatedandinvolvesmanycomponents whenfoldinggoeswrong it tellsthe bacteria thatthere is trouble envelope stress response. Essentialfortheformationofsomeproteins used for proteinscontainingcysteines butnotall cysteines are involvedindisulfide bonds theperiplasm is an oxidizing environment.
