BI226 Lecture Notes - Lecture 7: Small Nuclear Rna, Phosphodiester Bond, Pribnow Box

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7 Jul 2015
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Chapter 8: rna is abundant in cells and chemically similar to dna, rna in cytoplasm, where protein synthesis takes place, suggested that rna had a role in this. Rna: nucleotides composed of sugar, nitrogenous base, phosphate group, uracil is a base instead of thymine, the rest are the same, ribose is the sugar used rather than deoxyribose. It also forms the phosphodiester bonds between nucleotides. Identification of messenger rna (mrna: used to use pulse-chase technique to locate newly synthesized rna, pulse-chase experiment in bacteria. Examined transcription in bacteria by observing immediately after bacteriophage infection. Infection stimulated a quick burst of transcription, then it disappeared showing a rapid breakdown of rna. This led them to the conclusion that transcription was necessary for bacteriophage protein synthesis: experiments in eukaryotes. Made by protein-producing genes short-lived intermediate between dna and protein undergoes translation transcription of mrna is followed by post-transcriptional processing. Controls rna polymerase access to gene: coding region.

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