BI276 Lecture Notes - Lecture 7: Dna Gyrase, Semiconservative Replication, Novobiocin
Document Summary
Pork replication: diversity, flexibility complexity of their means of dna replication** Diagram: supercoiling action of dna gyrase (negative supercoiled) 3 stages of molecular information flow: 1. Replication: duplication of dna structure (dna template dictates the whole thing: 2. Transcription: dna participation in protein synthesis through rna: 3. What does euk have the proks do not- they ha(cid:448)e a (cid:374)u(cid:272)lear (cid:373)e(cid:373)(cid:271)ra(cid:374)e (cid:894)key differe(cid:374)(cid:272)e(cid:895)** that"s why dna must be super coiled in proks (dna gyrase in bacteria help super coil). Can have transcription and translation at the same time. Note: inhibit cell wall synthesis, dna gyrase (responsible for supercoiling dna) activity inhibited by quinolones, fluoroquinolones, novobiocin, archaea: reverse gyrases** they have something slightly different because have different structure. Recall: paired dna strands oriented in opposite directions relative to each other (like a zipper) one direction is continuous and discontinuous -lag strand, opposing direction, dna repli(cid:272)ated fro(cid:373) the 3" e(cid:374)d, dna replication in some bacteria is bidirectional.
