BI276 Lecture Notes - Lecture 1: Confocal Microscopy, Bright-Field Microscopy, Transmission Electron Microscopy
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Microbes: lecture 2: disproved theory of spontaneous generation. Robert hooke: (1635- 1703) english and discovered microorganisms (fungi: first to describe microbe. Illustrated the fruiting structures of mold. Antoni van leeuwenhoek: (1632- 1723) dutch: discovered bacteria. The postulates: the suspected pathogen must be present in all cases of the disease and absent from healthy animals. Tools: microscopy staining observe blood/tissue under the microscope: the suspected pathogen must be grown in pure culture. Tools: experimental animals: the suspected pathogen must be reisolated and shown to be the same as the original. Tools: laboratory reisolation and culture. The modern era of microbiology. Basic science sub- disciplines in microbiology. How to see very small objects chapter 5 page 123 (a brief journey to the microbial world) Types of microscopes: light microscopy. 1) preparing a smear: spread culture in thin film over slide dry in air, heat fixing and staining. Improving contrast in light microscopy. They become more pigmented: step 3: decolorization: ***
