BIOL 1000 Lecture 13: 10_3 Lecture 13
Document Summary
Enzymes require cofactors: metals, non protein organic molecules (coenzymes) Temp affects enzyme activity: all enzymes have different temps, humans. Denaturing protein: enzymes breaking down due to extreme temps, ph, salinity. Temp increases metabolic rate increases (to a limit) Enzymes begin to denature at temp above approx 40c. Rate of chemical reactions decrease and metabolic rate decreases. Ph level: pepsin level 2ish, glycogen phosphorylase level 6ish, trypsin level 11ish. Rate of reaction based on substrate and enzyme concentration. Rate will increase linearly w/ enzyme concentration. Inhibiting molecule binds to active site, prevents bonding of substrates. Inhibiting molecule binds to enzyme but not to active site, causing conformational change of active site. Inhibiting molecule covalently binds to enzyme but not active site. Non-comp binding to enzymes to control activity. Allosteric inhibition: enzymes binds allosteric inhibitor binding inhibitor converts enzyme to low-affinity state substrate released. Allosteric activation: enzyme binds allosteric activator binding activator converts enzyme to high-affinity state enzyme binds substrate.
