BIOL 2020 Lecture Notes - Lecture 6: Immunoglobulin Domain, Western Blot, Bohr Effect

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pearraven892

29 Apr 2016

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Cooperative binding: af nity of oxygen binding increases as more subunits are bound to o2. Conformation change (allosteric change) switches between the t (deoxy) and r form (oxy) There is an equilibrium between the t and r forms. The r form has more af nity for o2 than the t form. Equilibrium shifts towards the r form when o2 is already bound, thereby further increasing the af nity for more 02. 2,3-bpg binds the t form and enhances o2 release. The bohr effect: the regulation of oxygen binding by hydrogenous and carbon dioxide. Rapidly metabolizing tissues (ex. working muscle) produce high levels of h+ and co2. These act as allosteric effectors to promote o2 release in these tissues where it is needed. By binding to hb, h+ and co2: stabilize the deoxygenated form, destabilize the oxygenated form. Igg is the class that is highest in concentration in the blood serum.

Related Questions

Enzyme activity is decreased by:
repression of enzyme synthesis
proteolytic degradation of the enzyme
an increase in [product]
all of the above
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Which of the following are characteristics of the concerted allosteric model of Monod, Wyman, and Changeux?
a. there are at least two forms of the enzyme, termed T and R
b. there is an equilibrium between the T and R form
c. dimeric proteins can have one subunit in the T form and the other in the R form
d. the greater the L value (T/R), the more sigmoidal the v vs. [S] curve will be
e. regardless of the number of subunits in the enzyme, all must be in the T form or all in the R form
f. the level of cooperativity is greatest when KR >> KT
all but c
all but e
a, b, e, and f
a and b only
a, b, d, and e
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Which of the following are characteristics of the concerted allosteric model of Monod, Wyman, and Changeux?
a. there are at least two forms of the enzyme, termed T and R
b. there is an equilibrium between the T and R form
c. dimeric proteins can have one subunit in the T form and the other in the R form
d. the greater the L value (T/R), the more sigmoidal the v vs. [S] curve will be
e. regardless of the number of subunits in the enzyme, all must be in the T form or all in the R form
f. the level of cooperativity is greatest when KR >> KT
all but c
all but e
a, b, e, and f
a and b only
a, b, d, and e
------------------------------------------
Both hemoglobin and myoglobin share all of the following except:
they provide a protein framework that prevents heme-heme interactions with formation of Fe(III)
they are dimers of dimers with few Î±-Î± or Î²-Î² interactions
they non-covalently bind one heme per globin chain
they have a distal His that sterically hinders CO binding
-------------------------------
A graph of v versus [S] for an enzyme gives a hyperbolic plot. Which one of the following situations cannot give such a plot:
the enzyme obeys Michaelis-Menten kinetics
the enzyme model is an allosteric V system
the enzyme demonstrates positive cooperativity and is in the presence of an activator
the enzyme follows the Monod, Wyman, Changeux model and is in the presence of an inhibitor
All of the above would give a hyperbolic plot

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Which of the following is something that myoglobin and hemoglobin do not have in common?
oxygenation alters the quaternary structure of both
they both have oxygen bound to the heme at a 120Â° angle
they are both organized into regions of Î± helices
they both have two critical histidines that are crucial to their function
they both contain heme

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BIOL 2020 Lecture Notes - Lecture 6: Immunoglobulin Domain, Western Blot, Bohr Effect

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