BIOL 2021 Lecture Notes - Lecture 11: Reaction Rate, Reaction Rate Constant, Hyperbola
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Enzyme kinetics: the rate of the reaction catalyzed by enzyme e. A + b p is defined as. T: a and b changes are negative because the substrates are disappearing, p change is positive because product is being formed, enzyme activity can be assayed in many ways. Appearance of product: for example, you could measure. Appearance of colored product made from an uncolored substrate. Appearance of a uv absorbent product made from a non-uv-absorbent substrate. The velocity (v) of an enzyme-catalyzed reaction is dependent upon the substrate concentration [s] Velocity related to [s: a plot of v vs [s] is often hyperbolic. Graph is not a graph of product formation over time!! At this point, the enzyme is saturated with substrate, s. Rate at which cars can get through the booths is not affected by the number of waiting cars, only by the available number of toll attendants.