BIOL 2021 Lecture Notes - Lecture 8: Calnexin, Protein Folding, Mannose
7 Apr 2016
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2 pieces or 1 both sides of cheat sheet. Asymmetry of protein in plasma membrane (pm) Protein disulfide isomerize (pdi) an enzyme that converts sh to s-s. Protein glycosylation- sugars are added to protein. Lipid = dolichol (carries oligosaccharides) structure formed using couple specific sugars transferred to asn on protein core oligosaccharide. Sugar trimmed off in er and there. More modifications to the sugars are going to happen in golgi. Misfolded proteins are exported out of er destroyed by proteasome in cytosol. If folded exits er through the normal pathway. If not folded binds to glucosyl transferase. 6: another glucose added, binds to calnexin again cycle repeats itself (but you want it to stop eventually) Mannose trimming acts as = a timer (how much mannose is left) If the protein is too long there is too much mannose trimmed off. If that happens then the unfolded protein exported to cytosol (instead of the normal pathway)
