BIOL 2021 Lecture Notes - Lecture 17: Origin Recognition Complex, Cdc20, Sister Chromatids
13 Apr 2016
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17-11: will know the steps by the end. 17-15: anaphase-promoting complex (cyclosome) = apc/c; it is a ubiquitin ligase, which transfers ubiquitin to a protein = destruction. Targets (what it marks for destruction): securing (if you destroy securing at anaphase, it allows for the separation of the chromatids), cyclins s-cyclin and. M-cyclin (turns off cdks, which completes m phase) Activate apc via: cdc20 (activates it mid-mitosis), cdh1 (keeps it active during late mitosis and early g1) Changes in cyclin gene expression some cells; mostly g1/s + s cyclins. Table 17-2: we will be looking at wee1 kinase, cdc25, apc, cdc20, cdh1. Must copy dna once and only once! Dna synthesis = initiation at origin + elongation. Initiation 2 stages: (1) licensing in late m or early g; pre-replicative complex (pre-rc) assemble at origins of replication); pre-rc includes dna helicases that unwind dna (2) initiation beginning of s phase; activated s-
