BIOL 3110 Lecture Notes - Sanger Sequencing, Dna Replication, Restriction Enzyme
Document Summary
In order to understand the basic structure of dna it is important to understand the prevalent primary and secondary structure of it. Protein is the main component of dna both in terms of incorporation and in the construction of the molecule; the first protein to be sequenced is insulin. Dideoxy-nucleotide chain termination method is one of the oldest ways to sequence a protein, but it is considered old and inefficient compared to modern ways of sequencing (7. 12 singer & burk) The l isoform of amino acids are the ones that are used in proteins, not d. In dna replication there needs to be a template strand to indicate the specificity of which amino acid to insert along with other chemicals. If you want to stop the synthesis process you can use ddatp since it is missing. 3"oh, because there in no oh to attach the next nucleotide to.
