BIOL 3110 Lecture Notes - Plasmid, Recombinant Dna, Cloning
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Sept 29, 2009
Formation of a Recombinant DNA Genome
-A stretch of mammalian DNA is first sliced up by restriction enzymes to create
minor fragments for cloning. The fragments can be inserted into bacterial
fragments via hybridization (DNA-ligase base pairing) and then cloned by PCR.
-The human genome can generate around 1 million recombinant DNA molecules.
-Once the recombinant DNA molecules have been successfully hybridized onto a
bacterial plasmid it needs to be plated onto a gel for growth. The hybrids are
sparsely placed onto a gel and then the desired gene can be selected for.
-Transfer of DNA recombinants is usually inefficient and only a small portion of
the population will accept the hybridized gene.
***refer to previous notes for screening process***